DBCO-PEG4-Amine
Dibenzylcyclooctyne-PEG4-Amine
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
CLK-A103P-25 | 25 mg | Sob demanda | Adicionar ao Carrinho |
CLK-A103P-100 | 4 x 25 mg | Sob demanda | Adicionar ao Carrinho |
CLK-A103P-500 | 500 mg | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped at ambient temperature
Condições de armazenamento: store at -20 °C
store undissolved, for use prepare a fresh solution
Validade: 12 months after date of delivery (undissolved)
Fórmula molecular: C29H37N3O6
Peso molecular: 523.63 g/mol
CAS#: 1255942-08-5
Pureza: ≥ 90 % (HPLC)
Forma: oil
Solubilidade: DCM, DMF, DMSO, THF
Formulários:
Protein-peptide conjugates
Peptide-small molecule conjugates
18F radiolabelling
Protein-oligonucleotide conjugates
Surface modification
Descrição:
Unique carbonyl/carboxyl reactive dibenzylcyclooctyne, which reacts with acids, active esters and aldehydes. The very hydrophilic PEG4 spacer enhances solubility in water as well as in commonly used organic solvents of moderate polarity. Furthermore, the very water soluble, hydrophilic spacer reduces non-specific binding. Due to its length, the 24 Å (20 atoms) long spacer gives ready accessibility to the dibenzylcyclooctyne for the azide binding.
Important Product Information
Do not use DTT, TCEP or β-mercaptoethanol, because they will reduce the azide.
Copper-free Click ReactionPrepare the azide-containing sample in reaction buffer.Add DBCO-protein conjugate to azide-containing sample.Recommendation: Add 1 mol equivalent of limiting protein to 1.5 - 3.0 mol equivalents of highest abundance protein.Incubate the reaction at room temperature for 2 - 4 hours or at 4 °C for 2 - 12 hours.The reaction is now ready for purification.
Troubleshooting
Problem: Low conjugation of DBCO and azidePossible reason: Suboptimal reaction conditions
- Increase incubation time
- Optimize conjugation conditions by altering molar excess
- Perform conjugation reactions at 37 °C
Referências selecionadas:
Simon et al. (2012) Facile Double-Functionalization of Designed Ankyrin Repeat Proteins using Click and Thiol Chemistries. Bioconjugate Chem. 23 (2):279.
Zeng et al. (2012). 64Cu Core-Labeled Nanoparticles with High Specific Activity via Metal-Free Click Chemistry. ACS Nano. 6 (6):5209.
Arumugam et al. (2011). [18F]Azadibenzocyclooctyne ([18F]ADIBO): A biocompatible radioactive labeling synthon for peptides using catalyst free [3+2] cycloaddition. Bioorg. Med. Chem. Lett. 21:6987.
Campbell-Verduyn et al. (2011). Strain-Promoted Copper-Free Click Chemistry for 18F Radiolabeling of Bombesin. Angew. Chem. Int. Ed. 50:11117.
Debets et al. (2010) Aza-dibenzocyclooctynes for fast and efficient enzyme PEGylation via copper-free (3+2) cycloaddition. Chem. Commun. 46:97.