Clonagem livre de Ligase
![](/images/67ba7af28f/PP-110_Cloning_Kit.jpg)
The Ligase-free Cloning Kit generates plasmids usable for transformation in only two simple steps:
1) Creation of a PCR product with ends homologous to the cloning vector.
2) Annealing and fusion of insert and vector in a circularization reaction.
No time consuming ligation step is necessary!