dNTP set 100 mM
4 x 100 mM (dATP, dCTP, dGTP e dTTP)
2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
NUC-102S | 4 x 250 μL | 446,25 | Adicionar ao Carrinho |
NUC-102M | 4 x 400 μL | 714,00 | Adicionar ao Carrinho |
NUC-102L | 4 x 1000 μL | 1.610,70 | Adicionar ao Carrinho |
For in vitro use only!
Envio: shipped on blue ice
Condições de armazenamento: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Validade: 12 months after date of delivery
Fórmula molecular:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dTTP: C10H17N2O14P3 (free acid)
Peso molecular:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)
Pureza: ≥ 99 % (HPLC)
Forma: clear aqueous solution
Concentração: 100 mM - 110 mM
pH: 8.5 ±0.2 (22 °C)
Propriedades espectroscópicas: dATP: λmax 259 nm, ε 15.4 L mmol-1 cm-1 (Tris-HCl pH 7.0) dCTP: λmax 271 nm, ε 8.9 L mmol-1 cm-1 (Tris-HCl pH 7.0) dGTP: λmax 252 nm, ε 13.7 L mmol-1 cm-1 (Tris-HCl pH 7.0) dTTP: λmax 262 nm, ε 9.6 L mmol-1 cm-1 (Tris-HCl pH 7.0)
Descrição:
dNTP Bundle contains four separate solutions of ultrapure dATP, dCTP, dGTP and dTTP supplied as clear aqueous solutions (pH 8.5).
dNTP | Cat. No. | cap color |
dATP | NU-1001 | red |
dCTP | NU-1002 | blue |
dGTP | NU-1003 | yellow |
dTTP | NU-1004 | green |
Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable
Referências selecionadas:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.