SalI
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
EN-130S | 2.000 Units | Sob demanda | Adicionar ao Carrinho |
EN-130L | 5 x 2000 Units | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Definição de unidade: One unit is the amount of enzyme required to completely digest 1 μg of Lambda DNA (HindIII digest, 2 sites) in 1 hour in a total reaction volume of 50 μl. Enzyme activity was determined in the recommended reaction buffer.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles
Validade: 12 months
Forma: liquid (Supplied in 10 mM Tris-HCl pH 7.4, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 300 μg/ml BSA and 50 % [v/v] glycerol)
Concentração: 10 units/μl
Source: Streptomyces albus G Supplied with: 10x Universal Buffer (UB) Recommended 50 μl assay10 μl*||10x Universal Buffer (UB) 1 μg||pure DNA1 or PCR product2 5 units||enzyme fill up to 50 μl||PCR grade water * Optimum buffer condition for restriction is 2x UB 1 Supercoiled or high molecular weight DNA (e.g. plant genomic DNA) may require longer incubation time or higher amount of enzyme. 2 Some enzymes may require additional DNA bases flanking the restriction site for complete digestion. Protocol:The enzyme should not exceed 10 % of total reaction volume.Add enzyme as last component. Mix components well before adding enzyme. After enzyme addition, mix gently by pipetting. Do not vortex.Incubate 60 min. at 37 °C.Stop reaction by alternatively: - Addition of 2.1 μl EDTA pH 8.0 [0.5 M], final 20 mM - Heat Inactivation (20 min. at 65 °C) - Spin Column DNA Purification (e.g. PCR Purification Kit, Cat.-No. PP-201S/L) - Gel Electrophoresis and Single Band Excision (e.g. Agarose Gel Extraction Kit, Cat.-No. PP-202S/L) - Phenol-Chloroform Extraction or Ethanol Precipitation. Double Digestion - Buffer Compatibility: B1 -
Produtos relacionados: Universal Restriction Buffer, #EN-300