ScaI
Isoschizomers: Acc113I, ZrmI
JBSpeed Restriction Enzyme
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
EN-131S | 1.200 Units | Sob demanda | Adicionar ao Carrinho |
EN-131L | 5 x 1200 Units | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Definição de unidade: One unit is the amount of enzyme required to completely digest 1 μg of Lambda DNA (5 sites) in 1 hour in a total reaction volume of 50 μl. Enzyme activity was determined in the recommended reaction buffer.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles
Validade: 12 months
Forma: liquid (Supplied in 10 mM Tris-HCl pH 7.4, 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 μg/ml BSA and 50 % [v/v] glycerol)
Concentração: 10 units/μl
Source: Streptomyces caespitosus Supplied with: 10x Universal Buffer (UB) Recommended 50 μl assay5 μl||10x Universal Buffer (UB) 1 μg||pure DNA1 or PCR product2 10 units||enzyme fill up to 50 μl||PCR grade water 1 Supercoiled or high molecular weight DNA (e.g. plant genomic DNA) may require longer incubation time or higher amount of enzyme. 2 Some enzymes may require additional DNA bases flanking the restriction site for complete digestion. Protocol:The enzyme should not exceed 10 % of total reaction volume.Add enzyme as last component. Mix components well before adding enzyme. After enzyme addition, mix gently by pipetting. Do not vortex.Incubate 5 to 10 min. at 37 °C.Stop reaction by alternatively: - Addition of 2.1 μl EDTA pH 8.0 [0.5 M], final 20 mM - Heat Inactivation (20 min. at 80 °C) - Spin Column DNA Purification (e.g. PCR Purification Kit, Cat.-No. PP-201S/L) - Gel Electrophoresis and Single Band Excision (e.g. Agarose Gel Extraction Kit, Cat.-No. PP-202S/L) - Phenol-Chloroform Extraction or Ethanol Precipitation. Double Digestion - Buffer Compatibility: B1 - 90% of the DNA fragments can be ligated and recut with this enzyme. DNA Methylation: No Inhibition: dam, dcm, CpG Quality Control: All preparations are assayed for contaminating endonuclease, 3'-exonuclease, 5' exonuclease/ 5' phosphatase, as well as nonspecific single- and doublestranded DNase activities.
Produtos relacionados: Universal Restriction Buffer, #EN-300