NBD-GPP
3,7-dimethyl-8-(7-nitro-benzo[1,2,5]oxadiazol-4-ylamino),-octa-2,6-diene-1- pyrophosphate
Fluorescent lipid donor for protein farnesyltransferase (FTase)
Catálogo Nº | Apresentação | Preço (R$) | Comprar / Observação |
---|---|---|---|
LI-014 | 20 μl | Sob demanda | Adicionar ao Carrinho |
For general laboratory use.
Envio: shipped on gel packs
Condições de armazenamento: store at -20 °C
avoid freeze/thaw cycles, store dark
Validade: 12 months
Fórmula molecular: C16H22N4O10P2
Peso molecular: 492.31 g/mol
Pureza: ≥ 95 % (HPLC)
Forma: liquid (Supplied as 1 mM solution in 25 mM (NH4)2CO3)
Concentração: 1 mM
Propriedades espectroscópicas: λexc 480 nm, λem 530 nm, ε 11.0 L mmol-1 cm-1 (pH 7.5)
Descrição:
NBD-GPP is a fluorescent analog of geranyl pyrophosphate (GPP). It serves as lipid donor for farnesyltransferase (FTase) - one of the three mammalian protein prenyltransferases. The attachment of the NBD-geranyl group to protein substrates allows for efficient fluorescence based FTase activity assays and inhibitor screening.
Referências selecionadas:
Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227. Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704. Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822.