SYBR® Green Fluorescent DNA Stain
Corante intercalante de DNA para detecção de fluorescência em tempo real qPCR
Catálogo Nº | Apresentação | Preço (R$) | Comprar |
---|---|---|---|
PCK-303S | 500 μL | 317,10 | Adicionar ao Carrinho |
PCK-303L | 5 x 500 μL | 1.431,15 | Adicionar ao Carrinho |
Excitation (left) and emission (right) spectra of SYBR Green bound to dsDNA.
For in vitro use only!
Envio: Shipped on blue ice
Condições de armazenamento: Store at -20 °C
Store in the dark
Validade: 12 months
Forma: Orange liquid, supplied in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
Concentração: 100 μM
Propriedades espectroscópicas: λexc 495 nm (bound to DNA); λem 520 nm (bound to DNA)
Descrição:
SYBR® Green Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration. Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended. Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay. Please note that the
preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors. Select the optical setting for SYBR® Green or FAM on the detection instrument.
SYBR® Green Fluorescent DNA Stain is supplied as 100 μM concentration.
Vortex SYBR® Green Fluorescent DNA Stain thoroughly prior to its use. An SYBR® Green concentration of 0.5-1.0 μM in the final assay is recommended.
Add SYBR® Green Fluorescent DNA Stain as indicated in the table below per assay.
Please note that the preparation of a master mix may be crucial in quantitative PCR reactions to reduce pipetting errors.
Select the optical setting for SYBR® Green or FAM on the detection instrument.
Final Sybr® Concentration | 20 μl assay | 50μl assay |
0,5 μM | 0,1 μl | 0,25 μl |
1,0 μM | 0,2 μl | 0,5 μl |
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.