Kits para Síntese de mRNA
Visão geral das configurações dos kits de síntese de RNA
Produtos
HighYield T7 RNA Synthesis Kit RNT-101
RNA synthesis via in vitro transcription with T7 RNA Polymerase
HighYield T7 ARCA mRNA Synthesis Kit (m5CTP/Ψ-UTP) RNT-103
Synthesis of ARCA-capped, 5-methylcytidine & pseudouridine-modified (m)RNA
HighYield T7 mRNA Synthesis Kit (m5CTP/Ψ-UTP) RNT-104
Synthesis of 5-methylcytidine and pseudouridine-modified (m)RNA
Poly(A) Tailing Enzyme Testkit RNT-004
in vitro Polyadenylation of (m)RNA with E. coli and Yeast Poly(A) Polymerase
HighYield T7 ARCA mRNA Synthesis Kit (Ψ-UTP) RNT-114
Synthesis of ARCA-capped & pseudouridine-modified (m)RNA
HighYield T7 ARCA mRNA Synthesis Kit (me1Ψ-UTP) RNT-115
Synthesis of ARCA-capped & N1-Methylpseudouridine-modified (m)RNA
HighYield T7 ARCA mRNA Synthesis Kit (5moUTP) RNT-116
Synthesis of ARCA-capped & 5-methoxyuridine-modified (m)RNA
HighYield T7 ARCA mRNA Synthesis Kit (s2UTP) RNT-117
Synthesis of ARCA-capped & 2-thiouridine-modified (m)RNA
HighYield T7 ARCA mRNA Synthesis Kit (m5CTP) RNT-118
Synthesis of ARCA-capped & 5-methylcytidine-modified (m)RNA
HighYield T7 ARCA mRNA Synthesis Kit (ac4CTP) RNT-119
Synthesis of ARCA-capped & N4-acetylcytosine-modified (m)RNA
HighYield T7 ARCA mRNA Synthesis Kit (m6ATP) RNT-120
Synthesis of ARCA-capped & N6-methyladenosine-modified (m)RNA
HighYield T7 ARCA mRNA Synthesis Kit (m1ATP) RNT-121
Synthesis of ARCA-capped & N1-methyladenosine-modified (m)RNA
Referências Selecionadas
[1] Karikó et al.(2005) Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modification and the Evolutionary Origin of RNA. Immunity23:165.
[2] Karikó et al.(2008) Incorporation of Pseudouridine into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability. Mol. Ther.16(11):1833.
[3] Kormann et al.(2011) Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nature Biotechnology29(2):154.
[4] Warren et al.(2011) Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell7:618.
[5] Svitkin et al.(2017) N1-methyl-pseudouridine in mRNA enhances translation through eIF2alpha-dependent and independent mechanisms by increasing ribosome density. Nucleic Acid Res45(10):6023.
[6] Andies et al.(2015) N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. J. Control. Release217:337.
[7] Li et al.(2016) Effects of Chemically Modified Messenger RNA on Protein Expression. Bioconjugate Chem.27:849.
[8] Arango et al.(2018) Acetylation of Cytidine in mRNA Promotes Translation Efficiency. Cell175(7):1872.
[9] Sinclair et al.(2017) Profiling Cytidine Acetylation with Specific Affinity and Reactivity. ACS Chem. Neurosci.12(12):2922.
[10] Dominissini et al.(2016) The dynamic N1-methyladenosine methylome in eukaryotic messenger RNA. Nature530:441.
[11] Wienert et al. (2018) In vitro transcribed guide RNAs trigger an innate immune response via RIG-I pathway. PLoS Biol. 16 (7) :e2005840.
[12] Kim et al. (2018) CRISPR RNAs trigger innate immune responses in human cells. Genome Res. 28 (3):367.
[13] Badieyan et al. (2019) Concise Review: Application of Chemically Modified mRNA in Cell Fate Conversion and Tissue Engineering. Stem Cells Translational Medicine8:833.
[14] Hadas et al. (2019) Optimizing Modified mRNA In Vitro Synthesis Protocol for Heart Gene Therapy. Molecular Therapy: Methods & Clinical Development 14:300.
[15] Shatkinet al. (1976) Capping of eukaryotic mRNAs. Cell 9(4):645.
[16] Gallowayet al.(2019) mRNA cap regulation in mammalian cell function and fate. Biochimica et Biophysica Acta 1862(3):270.